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Study on Aqueous Two-phase Extraction of Xylanase

Guang Chen

Abstract

Orthogonal tests were designed to optimize the aqueous two-phase extraction process of xylanase from fermentation broth of Aspergillus niger AS3.4039 in this study. The results indicated that the optimum concentrations of PEG 4000, K2HPO4 and NaCl are 19%, 10% and 0 (W/W), respectively. Under these conditions, the separation factor (K) and xylanase yield in top phase (Yt) are 6.23 and 88.67% respectively, and the purification effect is satisfying.

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Key concepts: Xylanase, Extraction (chemistry), Aspergillus niger, Yield (engineering), Chemistry, Aqueous solution, Chromatography, PEG ratio