Analysis on clinical application of recombinant human luteinizing hormone for controlled ovarian hyperstimulation cycles in in vitro fertilization-embryo transfer

Objective:To evaluate the dose of human menopausal gonadotropin(HMG) added during late follicular phase in controlled ovarian hyperstimulation(COH) cycles of in vitro fertilization-embryo transfer(IVF-ET),and analyze the effect of recombinant human luteinizing hormone(r-hLH) supplement on main laboratory indexes and clinical pregnancy outcome.Methods:A total of two hundred and twelve patients treated with IVF-ET or intracytoplasmic sperm injection(ICSI) were selected,pituitary down-regulation standard long protocol was adopted,then all the patients were divided into two groups according to serum LH level during late follicular phase: experimental group(including seventy-two patients treated with r-hLH) and control group(including one hundred and forty patients treated without r-hLH);the indexes in the two groups were compared,including age,basic endocrine state,application time and dose of gonadotropin,endocrine hormones levels on the day of human chorionic gonadotropin(HCG) injection,the number of retrieved oocytes,2PN fertilization rate,high quality embryo rate,implantation rate,and clinical pregnancy rate.Results:There was no statistically significant difference in age,levels of basic LH,basic follicle stimulating hormone(FSH),and basic estradiol,days of COH,total dose of gonadotropin,2PN fertilization rate,implantation rate,and clinical pregnancy rate between experimental group and control group(P0.05).The total level of FSH in experimental group was statistically significantly lower than that in control group(P0.05);the total level of HMG in experimental group was statistically significantly higher than that in control group(P0.05);the level of serum LH on the day of HCG injection in experimental group was statistically significantly lower than that in control group(P0.05);the high quality embryo rate in experimental group was statistically significantly higher than that in control group(P0.05).Conclusion:For the patients with persistent low serum LH level during late follicular phase of COH cycles,increasing the dose of HMG and adding r-hLH reasonably can improve maturity of oocytes and significantly increase high quality embryo rate,which can improve the pregnancy outcome of patients receiving IVF/ICSI to a certain degree.