2009•Research and Practice on Chinese MedicinesRequires access

Study on HPLC Fingerprint of Rhizoma Curcumae Longae

Tian Mi

Abstract

Objective To establish the finger print of Rhizoma Curcumae Longae by HPLC. Methods The chromatographic conditions were as follows :Chromatographic column: Welchrom-C18,(250 mm×4.6 mm,5 μm), mobile phase: acetonitrile and water(gradient elution), flow rate: 0.8 mL/min, column temperature: 25 ℃ , detection wavelength: 270 nm. Results It was established the HPLC fingerprint common mode, and compared similiarity of Rhizoma Curcumae Longae from produce in different habitat and harvest time. Conclusion Each component was well separated, and can be used as a specif icity f ingerprint of Rhizoma Curcumae Longae.

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Key concepts: Chromatography, Chemistry, Gradient elution, High-performance liquid chromatography, Fingerprint (computing), Computer security, Computer science