The comparative study about biliary germiculture and antibiotic susceptibility test both in gallbladder and bile duct

Objective To investigate the biliary pathogenic bacteria and their susceptibility to antibiotics of bile from gallbladder and bile duct in biliary tract infection patients.Methods The bile from 273 patients with biliary tract infection during June 2005 and Oct.2007 were subjected to biliary gemiculture and sensitivity to antibiotics,and the results were statistically analyzed.Results The 149 patients with positive bile bacteria culture belonged to 17 of bile in gallbladder(19.8%,17/273)and 132 of bile in bile duct(61.7%,132/273).A total of 201 strains were identified,including 127 Gram negative strains(63.2%,127/201),74 Gram positive strains(36.8%,74/201),and no fungal strains were tested.The most common pathogenic bacteria were Escherichia coli(37.8%,76/201),Enterococcus(28.4%,57/201),Pseudomonas aeruginosa and Staphylococcus(each 8.5%,17/201).Drug sensitivity test indicated that Gram negative strains were sensitive to imipenem,meropenem,cefepime and amikacin,etc.,and so as Gram positive strains to vancomycin,teicoplanin and chloromycetin.Conclusion Gram negative strains remains the most common in biliary tract infection,but the rate of Gram positive is rising.The bacterio-infection rate of bile in bile duct is higher than in gallbladder.