Absorption and Separation of Red Pigments by Macroporous Resin from Pyracantha fortuneana

The extraction and purification technology of the red pigments from Pyracantha fortuneana is investigated by orthogonal design method.The preferable extraction conditions were as follows: solvent 80% ethanol,ratio of solvent to material 40,pH 3.0,temperature 50℃,time of extraction 3 h,the purification conditions for adsorption were X-5 macroporous resin,ratio of height to diameter of the resin loaded in the column 15,rate of elution 3 mL·min-1,pH3.0,concentration of the extract being separated 1 g·L-1.The amount of adsorption of the extract reached 0.037 15 g·mL-1 wet resin.The elution conditions were 95% ethanol as solvent,pH 2.0,elution rate 5 mL·min-1,3 times of the resin volume of eluent.