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CLONING OF HAL1 GENE FROM SACCHAROMYCES CEREVISIAE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR

Yin Yue

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Abstract

Taking Saccharomyces cerevisae Y2 genomic DNA as template, HAL1 gene was amplificated with PCR. Moreover the PCR product of HAL1 was cloned into pGEMT easy vector. The gene was sequenced and the results showed that the HAL1 gene contains 885 nucleotides and 99.6% identity with published sequence. The HAL1 gene was constructed on the plant expression vector pBY505.

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What this paper is about

Taking Saccharomyces cerevisae Y2 genomic DNA as template, HAL1 gene was amplificated with PCR. Moreover the PCR product of HAL1 was cloned into pGEMT easy vector. The gene was sequenced and the results showed that the HAL1 gene contains 885 nucleotides and 99.6% identity with published sequence. The HAL1 gene was constructed on the plant expression vector pBY505.

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Available abstract

Taking Saccharomyces cerevisae Y2 genomic DNA as template, HAL1 gene was amplificated with PCR. Moreover the PCR product of HAL1 was cloned into pGEMT easy vector. The gene was sequenced and the results showed that the HAL1 gene contains 885 nucleotides and 99.6% identity with published sequence. The HAL1 gene was constructed on the plant expression vector pBY505.

Key concepts: Gene, Cloning (programming), Genetics, Biology, genomic DNA, Expression vector, Saccharomyces cerevisiae, Nucleic acid sequence

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CLONING OF HAL1 GENE FROM SACCHAROMYCES CEREVISIAE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR — Research Paper | ScholarLens