CLONING OF HAL1 GENE FROM SCCHAROMYCES CEREVISIAE AS2.375 AND THE CONSTRUCTION OF PLANT EXPRESSION VECTOR OF HAL1 GENE

HAL1 gene is an important salt tolerant gene in yest.Total genomic DNA was isolated from Sccharomyces cerevisiae AS2.375.An about 900 bp DNA fragment was obtained with PCR technique by using the primer that designed according to the published sequence.The DNA fragment was cloned to T vector and transformed to E.coli strain JM109.The restriction map of the recombinant plasmid was analyzed and the DNA fragment was sequenced.The result showed that the entire open reading frame had been cloned and the identity of it's sequence to the published sequence is more than 99%.After cutting the HAL1 fragment from the T vector,we ligated the fragment to the pAM194 vector and obtained the plant expression vector of HAL1 gene.The transformants of tobacco with improved salt tolerant have been screened.