2009•Unpublished venueRequires access

Cloning of Hal1 gene and construction of its expression plasmid

Yuguang Chen

Abstract

Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template,Hal1 gene is amplified by PCR.The amplified product was digested with BamHI and EcoRI enzymes,then ligated into pYES2 vector.The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector.

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Available abstract

Key concepts: BamHI, EcoRI, Plasmid, Gene, Genetics, Vector (molecular biology), Restriction enzyme, Cloning vector