Effect of Lysis of Acupotomy on 5-HT,β-EP and Pathology in Rabbit with the Third Lumbar Vertebrae Transverse Process Syndrome

Objective:Observe the change of 5-HT,β-EP contents in serum and muscle tissue,and study the therapeutic mechanism of acupotomy lysis in rabbit with the third lumbar vertebrae transverse process syndrome.Methods:30 Japanese white rabbits were randomly divided into normal control group(NC),model control group(MC),acupotomy lysis group(AL),electroa-cupunture group(EA) and AL + EA group.5-HT,β-EP contents in serum and muscle tissue in rabbits were tested by Double Sandwich enzyme-linked immunoabsorbant assay(ELISA) technology on day 28.The H E staining was used to observe the pathological changes of local tissue.Results:Compared with normal control group,5-HT,β-EP contents in serum and muscle tissue were increased significantly In model control group.The contents of 5-HT in serum and muscle tissue and contents of β-EP in muscle tissue in 3 treatment groups were decreased significantly compared with model control group.HE staining:The in-flammation of local tissue in the model was severe,but in 3 treatment groups it lightened.Conclusion:Acupotomy lysis could medi-ate the peripheral analgesia effect by lightened inflammation and decreased the contents of 5-HT and β-EP contents.