Effect of Lysis of Acupotomy on Extracellular Matrix in Rabbit with Third Lumbar Vertebrae Transverse Process Syndrome

Objective:To observe the change of Collagen Ⅰand related metabolism enzyme in muscle tissuehomogenate in rabbit with the third lumbar vertebrae transverse process syndrome,and study the therapeutic mechanism of acupotomy lysis.Methods:30 Japanese white rabbits were randomly divided into normal control group,model group,acupotomy lysis(AL) group,electroacupunture(EA) group and AL+EA group.The model in rabbits with the third lumbar vertebrae transverse process syndrome was established by inserting a piece of gelatin sponge into the posterior of the left 3rd lumbar vertebrae transverse process.AL group and EA group are used acupotomy lysis and EA therapy applied to Wei Zhongof both sides,while AL+EA group used the two treatments.Col-Ⅰ,MMP-1,MMP-2 and TIMP-1,TIMP-2 contents in muscle tissuehomogenate in rabbits were tested by Double Sandwich enzyme-linked immunoab sorbant assay(ELISA) technology on day 28.Results:In model group,Col-Ⅰ,MMP-1,MMP-2 contents in muscle tissuehomogenate was increased significantly than normal while TIMP-1,TIMP-2 decreased significantly(P0.01),the contents of Col-Ⅰ,MMP-1,MMP-2 in AL group,EA group and AL+EA group were decreased and TIMP-1,TIMP-2 were increased,MMP-1 and TIMP-1 have statistically significant differences(P0.05).Conclusion:Acupotomy lysis can regulate the contents of Col-Ⅰ,MMPs and TIMPs in muscle tissuehomogenate,to maintain the dynamic balance between recombinant and degradation.