Construction of the Fused Eukaryotic Expression Vector of Mycobacterium Tuberculosis Rv2450 and Its Expression

To construct eukaryotic expression vector encoding Mycobacterium tuberculosis Rv2450, the gene encoding Rv2450 protein was amplified by polymerase chain reaction(PCR)from genome of Mycobacterium tuberculosis H37Rv strain.After sequencing the gene was then subcloned into eukaryotic expression vector pCDNA3.1(-). The recombinant plasmid pCDNA-Rv2450 was transfected into P815 cells with liposome. The expression was detected with RT-PCR and indirect immuno fluensent.Rv2450 was cloned into pCDNA3.1(-) correctly, and its expression was detected in P815 cells. Eukaryotic recombinant plasmids encoding Rv2450 were constructed successfully. The results established the basis for further study of the function of Rv2450.