Construction of the Fused Eukaryotic Expression Vector of Mycobacterium tuberculosis Rv1884 Gene and its Expression

Objective:To construct eukaryotic expression vector encoding Mycobacterium tuberculosis Rv1884 gene.Methods:The gene encoding Rv1884 protein were amplified by PCR from genome of M.tuberculosis H37Rv strain.After sequenced,Rv1884 gene segments were subcloned into eukaryotic expression vector pcDNA3.1(-).The recombinant plasmid pcDNA-Rv1884 were transfected into P815 cells with liposome.The expressions of mRNA and the expressed of proteins encoded by this gene were detected with RT-PCR and indirect immuno fluensent respectively.Results:Rv1884 gene was cloned into pcDNA3.1(-)correctly,and its expression was detected in P815 cells.Conclusion:Eukaryotic recombinant plasmids encoding Rv1884 were constructed successfully.The results established the basis for further study of the function of Rv1884.