CONSTRUCTION AND IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS Rv3873 FROM RD1 EUKARYOTIC EXPRESSION VECTOR

[Objective]To construct and identify the eukaryotic expression vector of Mycobacterium tuberculosis Rv3873. [Methods]Rv3873 was amplified by PCR and directly cloned into expression plasmid pGEX-4T-1 for sequencing. The target gene in the recombinant plasmid(pGEX-4T-1-Rv3873)was sub-cloned into the eukaryotic expression vector pcDNA3.1(+)and the recombinant pcDNA3.1-Rv3873. This recombinant plasmid was identified by restriction analysis and PCR.[Results]Comparison of the sequence of Rv3873 gene with that reported in GenBank showed that the identities were 100%.And it was comfirmed that Rv3873 gene was successfully inserted into pcDNA3.1(+).[Conclusion]We successfully construct the eukaryotic expression vector pcDNA3.1-Rv3873 and it lays a good foundation for the research of new DNA vaccine of Mycobacterium tubereulosis.