Microplate test of non-specific esterase of Cx.pipiens pallensfor insecticide resistance detection

Aim To explore simple and sensitive technic for insecticide resistance detection and early prediction Methods Microplate test was used to detect non specific esterase(NSE)of single Cx pipeins pallens of laboratory and field population,β Naphthyl acetate was used as the substrate and fast blue B salt solution as the colour agent Results The NSE level was highest in anti DDVP strain,followed by anti propoxur strain and anti DDVP degenerated strain,while the level was similar between anti cypermethrin and susceptible strain Three field collected Cx pipiens pallens populations have 4 15-9 36 fold higher resistance against DDVP and 1 02-3 81 fold higher resistance against propoxur than susceptible mosquito,and their NSE level was also higher than susceptible strain Conclusion Microplate test for NSE can be used for the discrimination of resistance against Op and carbamate insecticides The method is easy and rapid to operate with high precision and objective results Resistance frequency and mechanism can be detected at the same time,so early prediction of resistance and working out resistance management measure is possible