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STUDY ON THE ROLE OF NON-SPECIFIC ESTERASE OF CULEX PIPIENS PALLENS IN RESISTANCE AGAINST DIFFERENT INSECTICIDES

Li Shi

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Abstract

Objective To explore the role of non specific esterase (NSE) of Culex pipiens pallens in resistance against insecticides. Methods Laboratory detection of NSE was performed for 5 strains of the mosquito using β Naphthyl acetate as the substrate and fast blue B salt solution as the color agent. Results NSE level was highest in anti DDVP strain, followed by anti DDVP degenerated strain and anti propoxur strain, while the level of NSE of anti cypermethrin was similar to that of susceptible strain. Conclusion NSE was showed to play important role in the mosquito resistance against organophosphorus insecticides and was one of important mechanism for the development of resistance towards carbamates, while it has little relation with the resistance against pyrethroids.

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What this paper is about

Objective To explore the role of non specific esterase (NSE) of Culex pipiens pallens in resistance against insecticides. Methods Laboratory detection of NSE was performed for 5 strains of the mosquito using β Naphthyl acetate as the substrate and fast blue B salt solution as the color agent. Results NSE level was highest in anti DDVP strain, followed by anti DDVP degenerated strain and anti propoxur strain, while the level of NSE of anti cypermethrin was similar to that of susceptible strain. Conclusion NSE was showed to play important role in the mosquito resistance against organophosphorus insecticides and was one of important mechanism for the development of resistance towards carbamates, while it has little relation with the resistance against pyrethroids.

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Available abstract

Objective To explore the role of non specific esterase (NSE) of Culex pipiens pallens in resistance against insecticides. Methods Laboratory detection of NSE was performed for 5 strains of the mosquito using β Naphthyl acetate as the substrate and fast blue B salt solution as the color agent. Results NSE level was highest in anti DDVP strain, followed by anti DDVP degenerated strain and anti propoxur strain, while the level of NSE of anti cypermethrin was similar to that of susceptible strain. Conclusion NSE was showed to play important role in the mosquito resistance against organophosphorus insecticides and was one of important mechanism for the development of resistance towards carbamates, while it has little relation with the resistance against pyrethroids.

Key concepts: Propoxur, Esterase, Culex pipiens, Cypermethrin, Strain (injury), Insecticide resistance, Biology, Toxicology

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