MICROPLATE TEST OF NON-SPECIFIC ESTERASE OF CULEX PIPIENS PALLENS FOR INSECTICIDE RESISTANCE DETECTION

Objective To explore the simple and sensitive technic for insecticide resistance detection and early prediction. Methods Microplate test was used to detect non-specific esterase(NSE) of single Culex pipeins pallens of laboratory and field population, β-Naphthyl acetate was used as the substrate and fast blue B salt solution as the colour agent. Results The NSE Ievel was highest in anti-DDVP strain, followed by anti-propoxur strain and anti-DDVP degenerated strain, while the Ievel was similar between anti-cypermethrin and susceptible strain. Three field collected CJT. pipiens pallens populations had 4. 15 - 9. 36 fold higher resistance a-gainst DDVP and 1. 02 - 3. 81 fold higher resistance against propoxur than susceptible mosquito, and their NSE Ievel was also higher than susceptible strain. Conclusion Microplate test for NSE can be used for the discrimination of resistance against OP and carba-mate insecticides. The method is easy and rapid to operate with high precision and objective results. Resistance frequency and mecha-nism can be detected at the same time, so early prediction of resistance and working out resistance management measure is possible.