Reversal effect of Isorhynchophylline on cisplatin resistance of line A549/DDP human lung adenocarcinoma cells

Objective To determine the reversal effect of Isorhynchophylline(IR)on cisplatin resistance of human lung adenocarcinoma cell line A549/DDP.Methods The cytotoxic effect of IR-L and the sensitivity of cisplatin were determined by MTT assay.The intracellular concentration of cisplatin was detected by Inductively Coupled Plasma Mass Spectrometry(ICP-MS).Results The maximum non-toxic concentration group of IR(3.0 mg·L-1)increased the sensitivity of cisplatin in A549/DDP,decreased IC50 of cisplatin in A549/DDP from 16.81 mg·L-1 to 3.36 mg·L-1,and the IC50 of hyp-cytotoxic concentration(8.0 μg·mL-1)groups decreased to 2.34 mg·L-1 the intracellular concentration of cisplatin in A549/DDP cells;in IR groups significantly increased.Conclusion Isorhynchophylline can reverse the multidrug resistance(MDR)of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.