Effect of isorhynchophylline liposomes on cisplatin resistance of human lung adenocarcinoma cell line A549/DDP

Objective To determine the reversal effect of Isorhynchophylline Liposomes(IR-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.Methods The cytotoxic effect of IR-L and the sensitivity of cisplatin were determined by MTT assay.The intracellular concentration of cisplatin was detected by Inductively Coupled Plasma Mass Spectrometry(ICP-MS).Result The maximum non-toxic concentration group of IR-L(2.0 mg/L) increased the sensitivity of cisplatin in A549/DDP,decreased IC50 of cisplatin in A549/DDP from 16.81 mg/L to 3.36 mg/L,and the hyp-cytotoxic concentration(7.0 μg/mL) group's IC50 decreased to 2.34 mg/L;IR-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.Conclusion The results showed that Isorhynchophylline can reverse multidrug resistance(MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.