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2002•Journal of Modern RehabilitationRequires access

The effect of electric acupuncture on apoptosis in spinal cord injury

Zhi Zhang

Open publisher page 1 citations

Abstract

Objective To study the effect of electric acupuncture on apoptosis in spinal cord injury. Methods The rats were immediately treated in electric acupuncture after spinal cord injury(T10) by Allen's weight drop (50 g cm force) technique and killed at 6h and 24h after operation. The apoptosis of spinal slice was examined by the method of the terminal deoxynucleotidal transferase mediated dUTP biotin nick end labeling(TUNEL) reaction, the TUNEL positive cells were quantitatively analyzed by computer image analysis system. Result The number of TUNEL positive cells reduced significantly in spinal cord injured rats treated by electric acupuncture. Conclusion Electric acupuncture may prevent the progress of the second spinal cord injury by inhibits the apoptotic neuronal death after spinal cord injury.

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What this paper is about

Objective To study the effect of electric acupuncture on apoptosis in spinal cord injury. Methods The rats were immediately treated in electric acupuncture after spinal cord injury(T10) by Allen's weight drop (50 g cm force) technique and killed at 6h and 24h after operation. The apoptosis of spinal slice was examined by the method of the terminal deoxynucleotidal transferase mediated dUTP biotin nick end labeling(TUNEL) reaction, the TUNEL positive cells were quantitatively analyzed by computer image analysis system. Result The number of TUNEL positive cells reduced significantly in spinal cord injured rats treated by electric acupuncture. Conclusion Electric acupuncture may prevent the progress of the second spinal cord injury by inhibits the apoptotic neuronal death after spinal cord injury.

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Available abstract

Objective To study the effect of electric acupuncture on apoptosis in spinal cord injury. Methods The rats were immediately treated in electric acupuncture after spinal cord injury(T10) by Allen's weight drop (50 g cm force) technique and killed at 6h and 24h after operation. The apoptosis of spinal slice was examined by the method of the terminal deoxynucleotidal transferase mediated dUTP biotin nick end labeling(TUNEL) reaction, the TUNEL positive cells were quantitatively analyzed by computer image analysis system. Result The number of TUNEL positive cells reduced significantly in spinal cord injured rats treated by electric acupuncture. Conclusion Electric acupuncture may prevent the progress of the second spinal cord injury by inhibits the apoptotic neuronal death after spinal cord injury.

Key concepts: TUNEL assay, Spinal cord, Spinal cord injury, Acupuncture, Medicine, Apoptosis, Anesthesia, Pathology

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