The Effect of Electro-acupancture on PARP-1 of Cell Apoptosis after Spinal Cord Injury of Rats

Objective:With the model rats of acute SCI,the paper investigates that the electro-acupuncture affects the expression of neural cell apoptosis and changes related genes for PARP-1 after spinal cord injury of rats.The paper tries to show that the mechanisms of electro-acupuncture on spinal cord injure and the study of related important factors of mitochondrion excitement apoptosis way.Methods:Acute spinal cord injury models by Allen's preparation,the rats were divided into acupuncture group and drug group(Caspase-3 inhibitor group),model group,sham operation group.Apoptotic cells were marked by Tunel method.Results:Tunel labeling results showed that spinal cord injury after 6h,PARP-1 expression in the model group appeared,1d expression was the highest,on the 3 d,7 d it gradually decreased,on the 14 d it was the lowest.Model group expression levels were higher than that in sham operation group and drug group on the 1 d,3 d(P0.05).Expression of electro-acupuncture group was lower than that in model group on the 3 d(P0.05).Conclusion:First: Apoptosis occurs in rats after acute spinal cord injury.Spinal cord injury is the main pathological mechanism.Second: The expression of PARP-1 in rat neurons increased gradually after spinal cord injury,suggesting that PARP-1 may be involved in spinal cord injury.Third: Electro-acupuncture inhibits apoptosis of neural cells and reduces spinal cord injury by inhibiting neurons expression of PARP-1 in spinal cord injury.