Effects of nerve growth factor on expressions of apoptosis-related proteins in hippocampus of neonatal rats following hypoxic-ischemic brain damage

AIM: To investigate the effects of nerve growth factor (NGF) on the expression of apoptosis-related proteins (Bcl-2, Bax)in hippocampus of neonatal rats following hypoxic-ischemic brain damage (HIBD), and its molecular biological mechanism. METHODS: Seventy-two rats were randomly divided into control group, HIBD group and NGF group. The rat models of HIBD were established by modified RICE method. Immunohistochemistry and TUNEL staining were employed respectively to detect the expressions of Bcl-2 and Bax and the number of apoptotic hippocampal neurons. RESULTS: More or less apoptotic neurons could be observed in hippocampus in HIBD group and the level of Bcl-2 expression was elevated slightly, while Bax expression was significantly higher than that in control group. After NGF administration, the number of apoptotic neurons was decreased (P0.01), Bcl-2 expression increased (P0.01) and Bax expression decreased remarkably (P0.01) as compared with HIBD group. CONCLUSION: The administration of NGF may increase Bcl-2 expression and decrease Bax expression after HIBD in neonatal rats, thus inhibiting the apoptosis in hippocampus.