Study on the correlation between polymorphisms of p21 and p27 genes and the genetic susceptibility of non-small cell lung carcinoma in women

Objective To investigate the correlation between polymorphisms of p21 and p27 genes and the genetic susceptibility of non-small cell lung carcinoma(NSCLC)in women. Methods The single nucleotide polymorphisms(SNPs)in the 3'-untranslated region of p21 gene and in codon 109 of p27 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)in 110 NSCLC patients and 124 healthy subjects. Results The frequency of the p21 gene 3'UTR mutant genotypes ( C/T + T/T) in NSCLC patients(76. 4% )was significantly higher than that in healthy subjects(56. 5% ,P 0. 01). As compared with the C/C genotype,the genotypes with the 3'UTR T allele( C/T + T/T) significantly increased the susceptibility to NSCLC(age adjusted OR:2. 60,95% CI = 1. 46 ～ 4. 61). When stratified for smoking and aging,the frequency of combination of C/T and T/T genotype in non-smoking patients(86. 0% )and in patients whose age was above 50 years(76. 6% )were significantly higher than that in controls. The C/T and T/T genotype significantly increased the risk of pathogenesis of NSCLC in non-smokers(adjusted OR = 4. 95,95% CI = 2. 36 ～ 10. 40)and in group that patients'age was above 50 years(adjusted OR = 2. 68,95% CI = 1. 41 ～ 5. 08). There was no significant difference in overall genotype and allelotype distribution of the p27 gene variants between patients and controls,and nor did when stratified analyzing. There was no correlation between the lymph node metastasis and the two gene polymorphisms. Conclusion The research results suggest that in this population,the p21 gene 3 'UTR polymorphism is associated with NSCLC,C/T and T/T genotype may increase the risk of NSCLC,especially in nonsmokers and individuals whose age is above 50 years. The p27 gene V109G polymorphism may not be associated with the susceptibility of NSCLC. There is no correlation between the lymph node metastasis and the two gene polymorphisms.