Association of MSH5 gene C85T polymorphism with susceptibility of non-small-cell lung carcinoma

Objective To explore the association of MSH5 gene C85T polymorphism with the susceptibility of non-small-cell lung carcinoma(NSCLC). Methods The genotyping was performed by using the PCR-RFLP method in 107 NSCLC cases and 120 controls.Then,50%samples were selected randomly to be sequenced. Results The MSH5 gene C85T polymorphism,the frequencies of CC,CT and TT were 59.8%,35.5%,4.7%in the NSCLC group,and 78.3%,20.0%,1.7%in the normal control group,respectively.The frequency of the CT+TT genotype in NSCLC was significantly higher than that in the controls(χ2=9.170,P=0.002,OR=1.855,95%CI=1.229~2.798).The frequencies of the two alleles C and T were 77.6%and 22.4%in the NSCLC group,and were 88.3%and 11.7%in the normal control group,respectively.The T allele frequency was significantly higher in the NSCLC group than in the controls(χ2=9.405,P=0.002;OR=1.923,95%CI=1.253~2.950).The results of sequencing were completely consistent with those obtained by the PCR-RFLP method. Conclusions MSH5 C85T polymorphism is associated with the susceptibility of NSCLC significantly in a Chinese population.