Assosiation between matrix metalloproteinase 13 gene polymorphism and susceptibility to non-samall cell lung cancer

【Objective】To investigate the associations between-77A/G in the promoter region of MMP-13gene and susceptibilities of non-small cell lung cancer(NSCLC) in North China.【Methods】 This hospital-based case-control study included 300 non-small cell lung cancer patients(161 with squamous carcinoma and 139 with adenocarcinoma) and 300 healthy controls.Genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure.Polymorphisms of MMP-13 gene were analyzed by PCR-restriction fragment length polymorphism(RFLP).【Results】 The frequency of smokers in NSCLC patients(62.00%) was significantly higher than that in healthy controls(36.30%)(χ 2 =39.54,P =0.00).Smoking may increase the risk of developing NSCLC(age and gender adjusted OR =3.74,95% CI =2.451 ～5.705).The distribution of MMP-13-77A/G SNP in the promoter region genotypes among healthy controls did not significantly deviate from that expected by Hardy-Weinberg equilibrium(P 0.05).Frequency of the A allele in NSCLC patients was 50.33%,which was significantly higher than that in healthy controls 44.33%(χ2=4.332,P 0.05).The genotype distribution of the MMP-13-77A/G SNP in the overall NSCLC patients was also significantly different from that in healthy controls(χ 2 =8.638,P =0.013).Compared with G/ G genotype,the A/A genotype increased the risk of NSCLC(age,gender and smoking status adjusted OR=1.309,95% CI=1.033～1.659).Stratification analysis according to histological type,MMP-13-77A/G SNP in the promoter region showed significant statistical influence on the risk of adenocarcinoma.And comparing with G/G genotype,the A/A genotype could increase the risk of adenocarcinoma(age,gender and smoking status adjusted OR=1.512,95% CI=1.128～2.028).【Conclusions】 Smoking may increase the risk of developing NSCLC.The allele type and genotype distribution of the MMP-13-77A/G SNP in the overall NSCLC patients was significantly different from that in healthy controls.Compared with G/G genotype,the A/A genotype increased the risk of NSCLC,especially for adenocarcinoma.