Construction of the Recombinant Expression Plasmid pBI121-GFP-62390 and pBI121-GFP-51780 Contained Green Fluorescent Protein Gene

[Objective] The research aimed to construct the recombinant expression plasmid pBI121-GFP-62390 and pBI121-GFP-51780 contained green fluorescent protein gene.[Method] The GFP fragment was amplified by PCR.PCR production and pBI121 were double digested using BamH I+SacI.Big pBI121 fragment was purified from agarose gels.The plasmid was appraised through joint and transformation.[Result] A 740 bp fragment of green fluorescent protein gene was amplified by PCR.The fragment was cloned into pBI121 vector,and a new recombined vector was obtained.Arabidopsis' calmodulin genes At5g62390 and At3g51780 were cloned into the recombined vector pBI121-GFP.The new plant expression vector named pBI121-GFP-62390 and pBI121-GFP-51780 were constructed.The recombined plant expression vector were transferred into Agrobacterium tumefaciens strains of GV3101.[Conclusion] The study is a good start for the further research on the functions and location of genes At5g62390 and At3g51780.