Fan Wen
Abstract
The plant recombinant expression plasmid pBI GFP has been successfully constructed by cloning the green fluorescent protein gene which had been optimized by site directed mutagenesis into the plant expression vector pBI 121.The molecular biological technique such as single enzyme insertion was also used in this research.
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The plant recombinant expression plasmid pBI GFP has been successfully constructed by cloning the green fluorescent protein gene which had been optimized by site directed mutagenesis into the plant expression vector pBI 121.The molecular biological technique such as single enzyme insertion was also used in this research.
Key concepts: Green fluorescent protein, Recombinant DNA, Plasmid, Cloning (programming), Cloning vector, Mutagenesis, Molecular biology, Expression vector