Construction and identification of plant expression vectors containing HSP21.4 gene in Cyclamen

The experiment was conducted with HSP21.4 gene.Adding Sac I site to the endpoints of objective genes by using PCR amplification,then ligated with pBI121.The recombinant plasmid was transferred into competent cells of Escherishia coli DH5α.The PCR and Sac I digestion test showed that the HSP21.4 gene was constructed into plant expression vector,and recombinant plasmid designated as pBI-CpHSP21.4.The recombinant plasmid was transferred into competent cells of Agrohacterium tumefaciens LBA4404.One A.tumefaciens clone harboring recombinant plasmind pBI-CpHSP21.4 was obtained.It lays a foundation for testing function of HSP21.4 gene and transgenic plant expressing.