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2008•Journal of Oral and Maxillofacial SurgeryRequires access

Human Periodontal Ligament Stem Cells Differentiation into Osteoblasts in vitro

Hongwei Liu

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Abstract

Objective: To isolate and purify the human periodontal ligament stem cells(PDLSCs) and investigate the differentiation potentials of PDLSC into osteoblasts in vitro. Methods: PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103/cm2. At 80% confluence, the medium was changed to an inducing medium. After 21 days of induction, the results were evaluated by Alizarin red S staining, ALP activity test, immunohistochemistry and RT-PCR. Results: PDLSCs were cultivated and differentiated in vitro with active function, which were manifested by Alizarin red S staining and ALP activity test. Positive expression of collagenⅠ, BSP, ALP were confirmed by immunohistochemistry and RT-PCR. Conclusion: PDLSCs can be induced into osteoblasts in vitro. PDLSCs have the capability of multilineage differentiations.

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What this paper is about

Objective: To isolate and purify the human periodontal ligament stem cells(PDLSCs) and investigate the differentiation potentials of PDLSC into osteoblasts in vitro. Methods: PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103/cm2. At 80% confluence, the medium was changed to an inducing medium. After 21 days of induction, the results were evaluated by Alizarin red S staining, ALP activity test, immunohistochemistry and RT-PCR. Results: PDLSCs were cultivated and differentiated in vitro with active function, which were manifested by Alizarin red S staining and ALP activity test. Positive expression of collagenⅠ, BSP, ALP were confirmed by immunohistochemistry and RT-PCR. Conclusion: PDLSCs can be induced into osteoblasts in vitro. PDLSCs have the capability of multilineage differentiations.

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Available abstract

Objective: To isolate and purify the human periodontal ligament stem cells(PDLSCs) and investigate the differentiation potentials of PDLSC into osteoblasts in vitro. Methods: PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103/cm2. At 80% confluence, the medium was changed to an inducing medium. After 21 days of induction, the results were evaluated by Alizarin red S staining, ALP activity test, immunohistochemistry and RT-PCR. Results: PDLSCs were cultivated and differentiated in vitro with active function, which were manifested by Alizarin red S staining and ALP activity test. Positive expression of collagenⅠ, BSP, ALP were confirmed by immunohistochemistry and RT-PCR. Conclusion: PDLSCs can be induced into osteoblasts in vitro. PDLSCs have the capability of multilineage differentiations.

Key concepts: Periodontal ligament stem cells, ALIZARIN RED, Periodontal fiber, In vitro, Medicine, Staining, Immunohistochemistry, Stem cell

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