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Experimental research of inducing differentiation of PDLSCs by mineralization conditional medium

Hong Liu

Abstract

AIM:To isolate and purify the periodontal ligament stem cells from human third molars and investigate inducing differentiation of PDLSCs in mineralized medium.METHODS: PDLSCs were isolated by limited dilution of culture cells for single cell clone.PDLSCs were induced with minerralized conditional medium for 21 days.Differentiation of PDLSCs were assessed.Mineralized potential was studied by Von-Kossa staining.COLⅠ、COLШ、BSP、ON were examined by immunohistochemistry stains.RESULTS: PDLSCs formed in white calcified nodules by induced with minerralized conditional medium.The calcified nodules were stained by Von-Kossa.COLⅠ、COL Ⅲ、BSP、ON were positive stronger in the induces cells.CONCLUSION: PDLSCs could be induced to differentiate into osteoblasts in minerralized conditional medium in vitro culture.

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Key concepts: Periodontal ligament stem cells, Von Kossa stain, Staining, Chemistry, clone (Java method), In vitro, Cell biology, Biology