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2013•Chinese Journal of Practical StomatologyRequires access

Variation analysis of miRNA expression during osteogenic differentiation of human periodontal ligament stem cells

Guo Jiang-l

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Abstract

Objective To analyze the differential expression of miRNAs in periodontal ligament stem cells ( PDLSCs ) and osteo-differentiated PDLSCs. Methods PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103 / cm2. At 80% confluence , the medium was changed to an inducing medium. After 14 days of induction , the results were evaluated by Alizarin red S staining 、 ALP activity test and miRNA microarrays. Results PDLSCs were cultivated and differentiated in vitro with active function , which were manifested by Alizarin red S staining and ALP activity test. miRNA microarrays revealed that 13 miRNAs were downregulated in osteo-differentiated PDLSCs while 11 miRNAs were upregulated. Conclusions PDLSCs have the potential to differentiate into osteoblasts. miRNA may play an important role in the osteogenic differentiation of periodontal ligament stem cells.

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What this paper is about

Objective To analyze the differential expression of miRNAs in periodontal ligament stem cells ( PDLSCs ) and osteo-differentiated PDLSCs. Methods PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103 / cm2. At 80% confluence , the medium was changed to an inducing medium. After 14 days of induction , the results were evaluated by Alizarin red S staining 、 ALP activity test and miRNA microarrays. Results PDLSCs were cultivated and differentiated in vitro with active function , which were manifested by Alizarin red S staining and ALP activity test. miRNA microarrays revealed that 13 miRNAs were downregulated in osteo-differentiated PDLSCs while 11 miRNAs were upregulated. Conclusions PDLSCs have the potential to differentiate into osteoblasts. miRNA may play an important role in the osteogenic differentiation of periodontal ligament stem cells.

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Available abstract

Objective To analyze the differential expression of miRNAs in periodontal ligament stem cells ( PDLSCs ) and osteo-differentiated PDLSCs. Methods PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×103 / cm2. At 80% confluence , the medium was changed to an inducing medium. After 14 days of induction , the results were evaluated by Alizarin red S staining 、 ALP activity test and miRNA microarrays. Results PDLSCs were cultivated and differentiated in vitro with active function , which were manifested by Alizarin red S staining and ALP activity test. miRNA microarrays revealed that 13 miRNAs were downregulated in osteo-differentiated PDLSCs while 11 miRNAs were upregulated. Conclusions PDLSCs have the potential to differentiate into osteoblasts. miRNA may play an important role in the osteogenic differentiation of periodontal ligament stem cells.

Key concepts: Periodontal ligament stem cells, ALIZARIN RED, Periodontal fiber, Alkaline phosphatase, microRNA, Staining, Cell biology, Stem cell

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