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Assay of Vanillin by Reversed Phase -High Pressure Liquid Chromatography

Yan Guang-lie

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Abstract

A simple, rapid, accurate method is studied for assay of vanillin by Reversed Phase-High Pressure Liquid Chromatography. The chromatographic column is C18 with acetonitrile/water(85/15, V/V) as the eluting phase, and elute in isoconcentration. The detected wavelength is 279nm. The RSD is 0.46% and the recovery 98.17%～99.50%. The method is satisfactory for assaying the finished vanillin product, and also for analyzing the semifinished product .

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What this paper is about

A simple, rapid, accurate method is studied for assay of vanillin by Reversed Phase-High Pressure Liquid Chromatography. The chromatographic column is C18 with acetonitrile/water(85/15, V/V) as the eluting phase, and elute in isoconcentration. The detected wavelength is 279nm. The RSD is 0.46% and the recovery 98.17%～99.50%. The method is satisfactory for assaying the finished vanillin product, and also for analyzing the semifinished product .

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Available abstract

A simple, rapid, accurate method is studied for assay of vanillin by Reversed Phase-High Pressure Liquid Chromatography. The chromatographic column is C18 with acetonitrile/water(85/15, V/V) as the eluting phase, and elute in isoconcentration. The detected wavelength is 279nm. The RSD is 0.46% and the recovery 98.17%～99.50%. The method is satisfactory for assaying the finished vanillin product, and also for analyzing the semifinished product .

Key concepts: Vanillin, Chromatography, Chemistry, Acetonitrile, High-performance liquid chromatography, Elution, Phase (matter), Reversed-phase chromatography

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