3-MA on Adriamycin Induced K562 and K562 / ADM Cells Apoptosis and Related Gene mRNA Expression

Objective To investigate autophagy in cell apoptosis and mechanisms through the autophagy inhibitor 3-methyladenine( 3-MA) on the adriamycin( ADM) induced leukemia K562 cells and K562 / ADM cells effect and autophagy genes Beclin1,Survivin apoptosis inhibiting gene mRNA expression change of observation. Methods In vitro cultivation of K562 and K562 / ADM cells,Determined by MTT method was used respectively to detect the ADM and 3-MA pretreatment effect on K562 and K562 / ADM cells proliferation,Cell apoptosis was detected by flow cytometry,Real-time RT-PCR assay autophagy and apoptosis-related genes( Beclin1,survivin) mRNA expression changes. Results ADM inhibited K562 and K562/ADM cells proliferation,and the inhibition concentration and time dependence. In ADM induction group,K562 / ADM K562 cells apoptosis rate at 24 h,48 h,72 h were greater significantly than the control group( P 0. 05). Before the ADM induction,3-MA pretreatment could make the ADM-induced K562 and K562 / ADM cells inhibition rate and apoptosis rate were higher significantly than that induced with only ADM( P 0. 05). The relative expression levels of Beclin1,survivin mRNA decreased significantly when compared to only ADM induction( P 0. 05), with a positive correlation( r = 0. 827,P 0. 01). Conclusions ADM inhibited the growth of K562 and K562 / ADM cells,and induced cell apoptosis. 3-MA cells by inhibiting autophagy can enhance the ADM induced leukemia cell K562,K562 / ADM apoptosis. The mechanism may be associate to lower Beclin1 mRNA expression,and such suppressed the expression of survivin.