2008•Shandong yiyaoRequires access

Effect of Haishengsu on proliferation and expression of apoptosis gene in K562/ADM cells

Guangyao Li

Abstract

Objective To investigate the effect and mechanism of Haishengsu(HSS) on leukemia.Methods K562/ADM cells of leukemia were treated with HSS of different concentration.And the survival rates of K562/ADM cells was dectcted by MTT assay,the expression of Bcl-2 and Bax was dectcted by immunocytochemistry,the rate of apoptosis was dectcted by flow cytometer.the expression of caspase-3 protein was dected by western bolt.Results The profileration and expression of Bcl-2 of K56/ADM cells decreased,the apoptosis rate and expression of Bax increased after treated with HSS. Conclusion Hss can significantly inhibit the proliferation and induce apoptosis of K562/ADM cells.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: K562 cells, Apoptosis, Immunocytochemistry, Flow cytometry, MTT assay, Leukemia, Molecular biology, Chemistry