Trypsin Hydrolysis Pigskin Collagen and its Amino Acid Analysis

Pigskin collagen was hydrolyzed by Trypsin-chymotrypsin to explore the degrading pattern and the amino acid composition of the resultinghydrolysate.The results showed that heat Pigskin collagen 10 min at 95 ℃ was advantageous to enzymatichydrolysis of Pigskin collagen.Content of soluble nitrogen and amino nitrogen kept on increasing during hydrolysis,but increased slowly after 3 h hydrolysis.Peptide content reached a maximum after 3 h hydrolysis.A majority of unsoluble protein and peptides were degraded into soluble peptidesand amino acids after 6 h hydrolysis,acid composition in Pigskin collagen hydrolysate suggested that Pigskin collagen hydrolyzed by Trypsin-chymotrypsin improved the nutritional value of Pigskin collagen greatly.