Condition selection of the hydrolysis of pigskin with trypsin

In this work,trypsin was used to hydrolyze pigskin in order to avoid the destruction of collagen polypeptide during acid-alkali hydrolysis process.It was found that the hydrolysis degree increased with the increase of the trypsin content.The maximum hydrolysis degree of pigskin was realized at pH 7~8.The initial rate of the hydrolysis reaction was large while it decreased after reacting for 30 minutes.The optimum condition of the hydrolysis of pigskin by trypsin was as follows: pH 7.5,hydrolysis temperature 48 ℃,ratio of trypsin 40 mg/g,substrates concentration 8%,hydrolysis time 2 h.Under this condition,the hydrolysis degree of pigskin could reach 16%.