Apoptosis and expression of Bax,Bcl-2 and p53 in a rat of diabetes mellitus with cerebral ischemic reperfusion

Objective To observe the apoptosis and expression of Bax,Bcl-2 and p53 in a rat of diabetes mellitus with cerebral ischemic reperfusion.Methods Wistar rats were randomly divided into four groups: control group,sham operation group,ischemic reperfusion in hyperglycemia group(DIR) and normoglycemia group(NIR).Hyperglycemia model was made by injection of streptozocin through abdomen and model of cerebral ischemia reperfusion was developed by thread embolism method in rats.The apoptosis of cell and expression of Bax,Bcl-2 and p53 were detected by TUNEL and immunohistochemistry,respectively.Results Compared with sham operated and control group,expression of Bax,Bcl-2,p53 and percent of apoptosis were significantly higher in NIR group(P0.01);Compared with NIR group,expression of Bax,p53 and percent of apoptosis were significantly higher and expression of Bcl-2 were significantly lower in DIR group(P0.01).Conclusion Apoptosis which result from the change of Bax,p53 and Bcl-2 may play an important role in ischemic injury in diabetes rats with focal cerebral ischemia reperfusion.