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2004•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access

Effects of IGF-1 on apoptosis following cerebral ischemia and reperfusion in rats and its mechanism

Wang Jin-chun

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Abstract

Objective: To study the effects of IGF-1 on apoptosis and the expression of Bcl-2 and Bax following cerebral ischemia and reperfusion and evaluate the protecting mechanism of IGF-1. Methods: The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up. Thirty Wistar male rats were randomly divided into sham-operated group, ischemic group and IGF-1 treated group. IGF-1 of 10 μg was injected via caudal vein after 10 minutes with cerebral ischemia. The brain sections were used for TTC staining, TUNEL staining, Bcl-2 and Bax immunohistochemical staining. Results: Compared with ischemic group, the infarct volumes, the number of apoptotic cells and the expression of Bax positive cells in IGF-1 treated group were decreased significantly (P 0.01). The expression of Bcl-2 positive cells was increased significantly (P 0.01). Conclusions: IGF -1 can decrease apoptosis by up-regulating Bcl-2 and down-regulating Bax.

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What this paper is about

Objective: To study the effects of IGF-1 on apoptosis and the expression of Bcl-2 and Bax following cerebral ischemia and reperfusion and evaluate the protecting mechanism of IGF-1. Methods: The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up. Thirty Wistar male rats were randomly divided into sham-operated group, ischemic group and IGF-1 treated group. IGF-1 of 10 μg was injected via caudal vein after 10 minutes with cerebral ischemia. The brain sections were used for TTC staining, TUNEL staining, Bcl-2 and Bax immunohistochemical staining. Results: Compared with ischemic group, the infarct volumes, the number of apoptotic cells and the expression of Bax positive cells in IGF-1 treated group were decreased significantly (P 0.01). The expression of Bcl-2 positive cells was increased significantly (P 0.01). Conclusions: IGF -1 can decrease apoptosis by up-regulating Bcl-2 and down-regulating Bax.

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Available abstract

Objective: To study the effects of IGF-1 on apoptosis and the expression of Bcl-2 and Bax following cerebral ischemia and reperfusion and evaluate the protecting mechanism of IGF-1. Methods: The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up. Thirty Wistar male rats were randomly divided into sham-operated group, ischemic group and IGF-1 treated group. IGF-1 of 10 μg was injected via caudal vein after 10 minutes with cerebral ischemia. The brain sections were used for TTC staining, TUNEL staining, Bcl-2 and Bax immunohistochemical staining. Results: Compared with ischemic group, the infarct volumes, the number of apoptotic cells and the expression of Bax positive cells in IGF-1 treated group were decreased significantly (P 0.01). The expression of Bcl-2 positive cells was increased significantly (P 0.01). Conclusions: IGF -1 can decrease apoptosis by up-regulating Bcl-2 and down-regulating Bax.

Key concepts: Apoptosis, TUNEL assay, Ischemia, Immunohistochemistry, Medicine, Staining, Middle cerebral artery, Endocrinology

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Effects of IGF-1 on apoptosis following cerebral ischemia and reperfusion in rats and its mechanism — Research Paper | ScholarLens