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2013•Modern Chinese MedicineRequires access

Quality Standard of Danshen Shuxin Ning Capsules

Ren Cui-wen

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Abstract

Objective: To establish the quality standards of Danshen Shuxin Ning capsules.Methods: Studying on the qualitative identification of Salvia and red peony root by TLC,determinating salvianolic acid B content by HPLC,Thermo Scientific Hypersil BDS C 18 column( 250 mm × 4.6 mm,5 μm) was used,methanol-acetonitrile- Methanoicacid-water( 30:10:1:59 ) as mobile phase, the detection wavelength was 286 nm, flow rate was 1.0 mL·min-1.Results: Distinguishing the characteristic spots of Salvia and red peony root.The method is highly specific,and has good resolution.The linear range of salvianolic acid B was 0.016～0.144 mg·mL-1 ( r =1.000 0) , with an average recovery 97.79% ,RSD = 1.07%.Conclusion: The method is simple and feasible,and can be used for quality control of Danshen Shuxin Ning capsules.

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What this paper is about

Objective: To establish the quality standards of Danshen Shuxin Ning capsules.Methods: Studying on the qualitative identification of Salvia and red peony root by TLC,determinating salvianolic acid B content by HPLC,Thermo Scientific Hypersil BDS C 18 column( 250 mm × 4.6 mm,5 μm) was used,methanol-acetonitrile- Methanoicacid-water( 30:10:1:59 ) as mobile phase, the detection wavelength was 286 nm, flow rate was 1.0 mL·min-1.Results: Distinguishing the characteristic spots of Salvia and red peony root.The method is highly specific,and has good resolution.The linear range of salvianolic acid B was 0.016～0.144 mg·mL-1 ( r =1.000 0) , with an average recovery 97.79% ,RSD = 1.07%.Conclusion: The method is simple and feasible,and can be used for quality control of Danshen Shuxin Ning capsules.

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Available abstract

Objective: To establish the quality standards of Danshen Shuxin Ning capsules.Methods: Studying on the qualitative identification of Salvia and red peony root by TLC,determinating salvianolic acid B content by HPLC,Thermo Scientific Hypersil BDS C 18 column( 250 mm × 4.6 mm,5 μm) was used,methanol-acetonitrile- Methanoicacid-water( 30:10:1:59 ) as mobile phase, the detection wavelength was 286 nm, flow rate was 1.0 mL·min-1.Results: Distinguishing the characteristic spots of Salvia and red peony root.The method is highly specific,and has good resolution.The linear range of salvianolic acid B was 0.016～0.144 mg·mL-1 ( r =1.000 0) , with an average recovery 97.79% ,RSD = 1.07%.Conclusion: The method is simple and feasible,and can be used for quality control of Danshen Shuxin Ning capsules.

Key concepts: Chromatography, Quality standard, Chemistry, High-performance liquid chromatography, Salvia, Traditional medicine, Linear relationship, Content determination

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