Experimental observation on germicidal efficacy and toxicity of a compound hand disinfectant

Objective To understand the germicidal efficacy and safety performance of a new type compound hand disinfectant containing AKACID as the germicidal component.Methods The carrier quantitative germicidal test and the animal toxicity test were used to carry out the laboratory study.Results The kill rates of Escherichia coli and Staphylococcus aureus on cloth strips exposed to the stock solution of the disinfectant for 5 min and Candida albicans on cloth strip exposed for 10 min were 99.99%.After its stock solution in air tight condition was stored at 37 ℃ for 90 d,the germicidal efficacy was not affected.The bacteria suspension containing 50%(v/v) calf serum did not obviously influence its germicidal efficacy.Through on-the-spot disinfection test of 30 person′s hand,the compound disinfectant had good germicidal efficacy to natural bacteria of hands and was qualified for the kill ratio of natural bacteria.Its mouse oral LD50 was 5 000 mg/kg and its cumulative coefficient K was 5.The rabbit skin irritation score of its stock solution was 0 and eye irritation score was 3.0.The result of mouse bone marrow polychromatic erythrocyte micronucleus test for this disinfectant solution was negative and the sperm malformation test was also negative.Immersion in its stock solution for 72 h did not cause corrosion of stainless steel and copper and essentially did not cause corrosion of carbon steel and aluminum.Conclusion The compound disinfectant has good efficacy in killing vegetative forms of bacteria and fungi.It belongs to practically non-toxic class with weak cumulative toxicity and non-irritating to skin,non-irritating to eye.It is stable and low corrosive.