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2004•Chinese Journal of Neurosurgical Disease ResearchRequires access

Induced differentiation by matrine in C6 brain glioma cells

Liang Jingwen

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Abstract

Objective To investigate the effects of matrine on the proliferation of brain glioma C6 cell line in vitro.Methods Methyl thiagolyl tetragolium (MTT) was used to measure the levels of the proliferation of C6 cultured with matrine. The effect of matrine on cell cycle of C6 was observed by flow cytometer (FCM). The expression of glial fibrillary acidic protein (GFAP) of C6 cells was detected by immunohistochemistry in order to observing the condition of differentiation of C6 cells.Results The proliferation of C6 was obviously inhibited by matrine. The inhibitory rate was (24.4±0.9)%, when cultured with matrine at 0.5mg/ml. The outcome of FCM showed that the proliferation of C6 was obviously inhibited. The expression of glial fibrillary acidic protein (GFAP) was obviously enhanced in C6 cells cultured with matrine.Conclusion Matrine can inhibit the proliferation of C6 and induce differentiation of the cells in vitro.

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What this paper is about

Objective To investigate the effects of matrine on the proliferation of brain glioma C6 cell line in vitro.Methods Methyl thiagolyl tetragolium (MTT) was used to measure the levels of the proliferation of C6 cultured with matrine. The effect of matrine on cell cycle of C6 was observed by flow cytometer (FCM). The expression of glial fibrillary acidic protein (GFAP) of C6 cells was detected by immunohistochemistry in order to observing the condition of differentiation of C6 cells.Results The proliferation of C6 was obviously inhibited by matrine. The inhibitory rate was (24.4±0.9)%, when cultured with matrine at 0.5mg/ml. The outcome of FCM showed that the proliferation of C6 was obviously inhibited. The expression of glial fibrillary acidic protein (GFAP) was obviously enhanced in C6 cells cultured with matrine.Conclusion Matrine can inhibit the proliferation of C6 and induce differentiation of the cells in vitro.

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Available abstract

Objective To investigate the effects of matrine on the proliferation of brain glioma C6 cell line in vitro.Methods Methyl thiagolyl tetragolium (MTT) was used to measure the levels of the proliferation of C6 cultured with matrine. The effect of matrine on cell cycle of C6 was observed by flow cytometer (FCM). The expression of glial fibrillary acidic protein (GFAP) of C6 cells was detected by immunohistochemistry in order to observing the condition of differentiation of C6 cells.Results The proliferation of C6 was obviously inhibited by matrine. The inhibitory rate was (24.4±0.9)%, when cultured with matrine at 0.5mg/ml. The outcome of FCM showed that the proliferation of C6 was obviously inhibited. The expression of glial fibrillary acidic protein (GFAP) was obviously enhanced in C6 cells cultured with matrine.Conclusion Matrine can inhibit the proliferation of C6 and induce differentiation of the cells in vitro.

Key concepts: Matrine, Glial fibrillary acidic protein, In vitro, Chemistry, Glioma, Cell culture, Cell growth, Molecular biology

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