The Effects of Matrine on Proliferation of Line A549 with Lung Adenocarcinoma

Objective To investigate the effects of matrine on proliferation of line A549 with lung adenocarcinoma cultured in vitro.Methods The A549 cells were treated with matrine in different concentrations and variable durations.The effect of matrine on A549 cells proliferation inhibition,apoptosis promotion,F-actin location and Bcl-2 protein expression were observed by the methods of cell growth curves,XTT,Flow cytometry and Western-blot.Results Compared with the control group,Matrine at the concentration of 0.1 to 0.5mg·mL-1 had a significant time and dose-dependent effect on proliferation inhibition(P0.01).FCM detection showed matrine could induce apoptosis and increase expression of F-actin in A549 cell.The expression of bcl-2 protein was significantly decreased in the matrine groups by Western-blotting.Conclusion Matrine can efficiently inhibit the proliferation and induce apoptosis of A549 cell,down-regulate bcl-2 protein expression which are involved in its anti-cancer effect.