Inhibition of proliferation and induction of apoptosis of glioma cells by matrine

AIMTo investigate the effects of matrine on the proliferation of human glioma cell line BT325. METHODS MTT was used to measure the levels of the proliferation of BT325 cultured with matrine in different concentrations. The effects of matrine on cell cycle of BT325 were observed by FCM. The morphological change of BT325 cell was detected by electronic microscopy. RESULTS The proliferation of BT325 was obviously inhibited by matrine in a dose dependent manner. The inhibitory rate came to the peak at (32 1±1 1)%, when cultured with matrine at 0 5 g·L -1 . The outcome of FCM showed that the apoptotic cell rate was 23 9%. The morphological changes of apoptosis were also found by electronic microscopy. CONCLUSION Matrine can inhibit the proliferation of BT325 and induce the apoptosis of the cells.