Purification of total flavones from leaves of Chimonanthus nitens by macroporous adsorption resins

Objective To investigate the technological parameters of the purification process of total flavones from the leaves of Chimonanthus nitens with macroporous adsorption resins.Methods Taking the static elution ratio and static absorption ratio as indexes,the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index.Results The HPD400 macroporous resin among eight types of resins was found with the best separating efficiency,by which the dynamic absorption capacity could reach 1.68 g(crude drug)/g.Static absorption capacity and desorption ratio were 17.77 mg/g and 92.24%,respectively.After eluted with 4 BV of distilled water and 4 BV of 30% ethanol,eluant was changed with 6 BV of 70% ethanol,the yield of total flavones was 31.4% and product purity was 88.4%.Conclusion With definite technical process,the HPD400 macroporous adsorption resin could be used to purify the extract in the leaves of C.nitens.