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2007•Unpublished venueRequires access

Cloning and Sequence Analysis of IFN-Alpha in Gushi Chicken

Qian Guan

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Abstract

Genome DNA was extracted and then used to amplificate chicken interferon-alpha gene directly with primers which were designed according to the sequences of chicken Interferon-alpha on the GenBank.Specific fragments were linked to pGEM-T Easy vector,then transformed to competent cells of JM109.By idenfication of plasmid PCR and enzyme digestion,positive strain was sent to Takara for sequencing.The sequencing results showed that we have obtained Interferon-alpha gene of Gushi chicken.The results of homology analysis with other chicken interferon-alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 97.9%at nucleotide homology and that of 96.9% at amino acid homology .

About this research paper

What this paper is about

Genome DNA was extracted and then used to amplificate chicken interferon-alpha gene directly with primers which were designed according to the sequences of chicken Interferon-alpha on the GenBank.Specific fragments were linked to pGEM-T Easy vector,then transformed to competent cells of JM109.By idenfication of plasmid PCR and enzyme digestion,positive strain was sent to Takara for sequencing.The sequencing results showed that we have obtained Interferon-alpha gene of Gushi chicken.The results of homology analysis with other chicken interferon-alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 97.9%at nucleotide homology and that of 96.9% at amino acid homology .

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Available abstract

Genome DNA was extracted and then used to amplificate chicken interferon-alpha gene directly with primers which were designed according to the sequences of chicken Interferon-alpha on the GenBank.Specific fragments were linked to pGEM-T Easy vector,then transformed to competent cells of JM109.By idenfication of plasmid PCR and enzyme digestion,positive strain was sent to Takara for sequencing.The sequencing results showed that we have obtained Interferon-alpha gene of Gushi chicken.The results of homology analysis with other chicken interferon-alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 97.9%at nucleotide homology and that of 96.9% at amino acid homology .

Key concepts: GenBank, Biology, Gene, Homology (biology), Cloning (programming), Genetics, Sequence analysis, Alpha interferon

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