Cloning and Sequence Analysis of Interferon-Gene of Three Line Chickens

One pair of primers was designed according to chicken interferon-alpha(IFN-α)gene sequences retrieved from the GenBank.Chicken IFN-α genes were amplified from genome DNA extracted directionally from α livers of Guishi chicken,Hailan chicken and Luoman chicken.The purified PCR products were cloned into pGEM-T Easy vector and sequenced.The result showed that the full length sequence of chicken IFN-α gene was consisted of 582 bp,which includes one open-reading frame(582 bp),encoding 193 amino acid residues.When the nucleotide sequences of three line chicken IFN-α genes were compared,the nucleotide sequence of Hailan chicken IFN-α gene was just the same as Luoman chicken IFN-α gene,the nucleotide sequence of Guishi chicken IFN-α shared 99.3% homology with that of Hailan chicken and Luoman chicken IFN-α,and putative amino acid sequence shared 97.9%.When three line chicken IFN-α genes were compared with seven chicken IFN-α genes published previously in GenBank,there was identity of 98.1%~100%at nucleotide acid level,and that of 96.9%~100% separately at deduced amino acid level.