2004•ZhongchengyaoRequires access

Determination of astragaloside IV in Qixiu Granules by HPLC-ELSD

Tian Jin

Abstract

AIM: To determine astragaloside Ⅳ in Qixiu Granule (Radix Astragali seu Hedysari, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, etc.) by HPLC ELSD. METHODS: HPLC ELSD was used in the quantitative analysis by using Alltima C 18 chromatography column and acetonitril water (34∶66) as a mobile phase. The flow rate of mobile phase was 1mL·min -1 . The tube temperature of the detector was 100°C. The flow rate of pure air was 2.7L·min -1 . RESULTS: The regressione quation was Y=-4.40×105+2.07×105X, r=0.9992 (3.73～13.59μg). The average recovery of astragaloside Ⅳ was 100.73%, RSD was 3.97%. CONCLUSION: The method is simple, reliable, accurate and can be regard as the quantity control method of Qixiu Granule.

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Key concepts: Astragaloside, Chromatography detector, Drift tube, Chromatography, Chemistry, High-performance liquid chromatography, Granule (geology), Radix (gastropod)