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Determination of astragaloside IV in Qiluyishen Tabletes by HPLC-ELSD

Chen Yue-rong

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Abstract

AIM: To establish a method for the determination of Astragaloside Ⅳ in Oiluyishen Tabletes(Radix Astragali, Herba Pyrolae, Rhizoma Atractylodis Macrocephalae, Poria, Radix Codonopsis,etc.) by HPLC-ELSD. METHODS: Chromatography was performed on a 5μm HIQsilC 18 column(4.6mm×250mm) at 25℃. The mobile phase composition was acetonitrile-water(34∶66). The flow rate was at 1.0mL·min -1. An evaporation light-scattering detector (ELSD) was used as detector with gas flow rate of 2.6 mL·min -1 and drift tube temperature at 100℃. RESULTS: The linear range of Astragaloside Ⅳ was in the range of 2.288～ 22.88μg, r=0.999, the average recovery was 98.8% and RSD was 1.2%. CONCLUSION: This method is simple, feasible and reproducible. It can be used for the quality control of Oiluyishen Tabletes.

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What this paper is about

AIM: To establish a method for the determination of Astragaloside Ⅳ in Oiluyishen Tabletes(Radix Astragali, Herba Pyrolae, Rhizoma Atractylodis Macrocephalae, Poria, Radix Codonopsis,etc.) by HPLC-ELSD. METHODS: Chromatography was performed on a 5μm HIQsilC 18 column(4.6mm×250mm) at 25℃. The mobile phase composition was acetonitrile-water(34∶66). The flow rate was at 1.0mL·min -1. An evaporation light-scattering detector (ELSD) was used as detector with gas flow rate of 2.6 mL·min -1 and drift tube temperature at 100℃. RESULTS: The linear range of Astragaloside Ⅳ was in the range of 2.288～ 22.88μg, r=0.999, the average recovery was 98.8% and RSD was 1.2%. CONCLUSION: This method is simple, feasible and reproducible. It can be used for the quality control of Oiluyishen Tabletes.

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Available abstract

AIM: To establish a method for the determination of Astragaloside Ⅳ in Oiluyishen Tabletes(Radix Astragali, Herba Pyrolae, Rhizoma Atractylodis Macrocephalae, Poria, Radix Codonopsis,etc.) by HPLC-ELSD. METHODS: Chromatography was performed on a 5μm HIQsilC 18 column(4.6mm×250mm) at 25℃. The mobile phase composition was acetonitrile-water(34∶66). The flow rate was at 1.0mL·min -1. An evaporation light-scattering detector (ELSD) was used as detector with gas flow rate of 2.6 mL·min -1 and drift tube temperature at 100℃. RESULTS: The linear range of Astragaloside Ⅳ was in the range of 2.288～ 22.88μg, r=0.999, the average recovery was 98.8% and RSD was 1.2%. CONCLUSION: This method is simple, feasible and reproducible. It can be used for the quality control of Oiluyishen Tabletes.

Key concepts: Chromatography detector, Chromatography, Radix (gastropod), Drift tube, Astragaloside, Chemistry, High-performance liquid chromatography, Linear range

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