2008•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Icariin in Tongmai Qiangshen Wine by HPLC

Hong Li

Abstract

ObjectiveTo establish an HPLC method for determination of Icariin in Tongmai Qiangshen Wine.MethodsWith Discovery column C18,the mobile phase was acetonirile-0.025 mol/L phosphoric acid(25∶75).The detection wavelength was 270nm.ResultsThe icariin samples showed a good relationship within the range of 0.1344~0.896,r=0.999 9,the average recovery of add samples was 97.4,RSD was 1.60%.ConclusionThe method is simple and accurate.

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Key concepts: Icariin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Wine, Chemistry, Medicine, Food science