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HPLC Determination of Icariin in Bushen Qiangshen Tablets

WU Cha-qing

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Abstract

To determineation icariin in the Bushen Qiangshen tablets.Method:HPLC,Agilent XDB-C_(18) (4.6mm×150mm,5μm).The mobile phase was acetonitrile-water-glacialacetic acid(29:70:1),flow rate was 0.85mL·min~(-1).The determination wavelength was 270 nm.Results:The linear range was 0.73-3.64μg (r= 0.9999).The average recover was 100.5%,RSD=1.6% (n=6).Conclution:The method is simple and reliable with good repeatability and can be used for the quality control method for this tablets.According to the results of de- termination of icariin,the Bushen Qiangshen tablets from different manufactory in the markets shows much difference.

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What this paper is about

To determineation icariin in the Bushen Qiangshen tablets.Method:HPLC,Agilent XDB-C_(18) (4.6mm×150mm,5μm).The mobile phase was acetonitrile-water-glacialacetic acid(29:70:1),flow rate was 0.85mL·min~(-1).The determination wavelength was 270 nm.Results:The linear range was 0.73-3.64μg (r= 0.9999).The average recover was 100.5%,RSD=1.6% (n=6).Conclution:The method is simple and reliable with good repeatability and can be used for the quality control method for this tablets.According to the results of de- termination of icariin,the Bushen Qiangshen tablets from different manufactory in the markets shows much difference.

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Available abstract

To determineation icariin in the Bushen Qiangshen tablets.Method:HPLC,Agilent XDB-C_(18) (4.6mm×150mm,5μm).The mobile phase was acetonitrile-water-glacialacetic acid(29:70:1),flow rate was 0.85mL·min~(-1).The determination wavelength was 270 nm.Results:The linear range was 0.73-3.64μg (r= 0.9999).The average recover was 100.5%,RSD=1.6% (n=6).Conclution:The method is simple and reliable with good repeatability and can be used for the quality control method for this tablets.According to the results of de- termination of icariin,the Bushen Qiangshen tablets from different manufactory in the markets shows much difference.

Key concepts: Icariin, Chemistry, Chromatography, Repeatability, High-performance liquid chromatography, Linear relationship, Linear range, Detection limit

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