Comparison research of methods of DNA extraction from grape

Five methods,i.e. CTAB,high saltlow pH,basic method,high salt precipitation, improved SDS method, were used to extract total DNA from the young leaves of grape. The resulted DNA samples of five methods were tested qualitatively and quantitatively using the value of A260/A280 、agarose gel electrophoresis and RAPD. The results show that high salt precipitation and high saltlow pH methods are better as a whole. The purity of extracted DNA by improve SDS is the best. So we should select the proper method according to the objetive in the study.